Journal: Molecular Cancer

Article Title: CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment

doi: 10.1186/s12943-022-01658-x

Figure Lengend Snippet: CREB3L1 is associated with ECM signaling in thyroid cancer. A GSEA determined the enrichment differences of biological processes depending on the CREB3L1 expression. B-C The evaluated activity scores of the extracellular matrix (ECM) and collagen signaling in NT, PTC and ATC samples. D-E The Pearson correlation analysis of CREB3L1 and ECM or collagen signaling. F Immunofluorescence staining of CREB3L1 and fibroblast marker FAP in thyroid cancer samples. G RT - PCR was used to detect the expression of collagen signals after CREB3L1 knockdown. H The expression of COL5A1 was detected, after CREB3L1 knockdown or overexpression in thyroid cancer cell lines. I-J After CREB3L1 knockdown, Masson trichrome staining and IHC staining were used to detect changes in collagen fibril abundance and the expression of α - SMA after CREB3L1 knockdown, respectively. K Immunofluorescence staining of COL5A1 in ATC cell-derived xenografts after CREB3L1 knockdown. Data are shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 NC versus CREB3L1 - KD

Article Snippet: To analyze the α - SMA - , FAP - , or PDGFRα - positive CAFs, found in the 8505C - derived sphere or co - culture with 8505C, the samples were digested and resuspended in PBS, containing 1.5% BSA and incubated with the primary antibodies anti - α - SMA (Cat#14,395–1 - AP, Proteintech), anti - FAP (FAB3715A, R&D Systems, Minnesota, USA), and anti - PDGFRα (#567,950, BD biosciences), respectively.

Techniques: Expressing, Activity Assay, Immunofluorescence, Staining, Marker, Reverse Transcription Polymerase Chain Reaction, Knockdown, Over Expression, Immunohistochemistry, Derivative Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Radiation-induced FAP + fibroblasts are involved in keloid recurrence after radiotherapy

doi: 10.3389/fcell.2022.957363

Figure Lengend Snippet: Irradiation (IR) promoted FAP + KF proliferation and inhibited senescence, accompanied by high energy production. In vitro , primary KFs with or without IR treatment were analyzed by anti-hFAP and anti-hVimentin antibody staining (A) . Representative images of SA-β-gal staining (B) ; senescent cells are stained green, scale bar = 100 μm. The percentage of senescent cells in FAP- versus FAP + KFs at 72 h after IR (C) . Data are representative of three independent samples and are presented as the mean ± SD; n = 6; ****, p < 0.001. IR promoted FAP + KF proliferation (D) and increased intracellular ATP production (E) . Data from three experiments with essentially the same results were combined and are presented as the mean ± SD; n = 3; *, p < 0.05; **, p < 0.01; ****, p < 0.001.

Article Snippet: The cells were analyzed by staining with an AF488-conjugated anti hFAP antibody (R&D Systems, FAB3715G-100) and a PE-conjugated anti-hVimentin antibody (BD, 562337).

Techniques: Irradiation, In Vitro, Staining